Molecular Biology & Immunobiology of Streptococcus Mutans


Specific Objectives

To help understand the complex interactions in Streptococcus mutans-induced biofilm and caries formation, we are defining the role cell surface proteins play in colonization. Mechanisms that may diminish colonization of S. mutans cells to enamel surfaces are being explored.  The effect of tobacco on S.mutans visulence is a major focus.  Inhibitory factors being investigated include mucosal vaccines and neutralization of receptor sites.

Licensing and sponsored research opportunities abstracts:

  • Polypeptides Useful in the Prevention of Dental Caries

  • A Novel Assay for Determination of Dental Caries Susceptibility

Licensing and sponsored research opportunities contact Mark Reichel
For research collaboration contact Dr. Gregory

Program Director

Dr. Richard Gregory, Ph.D

Other Faculty Supporting the Program

Dr. L. Jack Windsor, Ph.D

Dr. Fengyu Song, D.D.S., M.S., Ph.D

Dr. Dominique Galli, M.S., Ph.D

Dr. Michael Kowolik, DDS, Ph.D

Dr. Ygal Ehrlich, D.M.D

Graduate Students Supporting the Program

Ruijie (Margaret) Huang
Mingyun (Ming) Li
Brandon Scheer

Recent Publications*

Papers Published in Refereed Journals

Xie, D., Y. Weng, X. Guo, J. Zhao, R.L. Gregory and C. Zheng.  2011.  Preparation and evaluation of a novel glass-ionomer cement with antibacterial functions.  Dent. Mat. (in press).

Allam, E., W. Zhang, C. Zheng, R.L. Gregory, and L.J. Windsor.  2011.  Smoking and oral health.  In: Cigarette Smoke Toxicity - Linking Individual Chemicals to Human Diseases.  D. Bernhard (ed.).  Wiley-VCH Verlag GmbH & Co. KGaA  (pub.)  pp. 257-280.

Huang, R., M. Li and R.L. Gregory.  2011.  Bacterial interactions in dental biofilm.  Virulence. (in press).

Weng, Y., X. Guo,J. Zhao, R.L. Gregory and D. Xie. 2010. A PQAS-containing glass-ionomer cement for improved antibacterial function.  J. Biomedical Science and Engineering. 3:956-963.

Weng, Y., X. Guo, R.L. Gregory and D. Xie. 2010. A novel antibacterial dental glass-ionomer cement.  Eur. J. Oral Sci. 118:531-534.

Sanui, T. and R.L. Gregory. 2009. Analysis of Streptococcus mutans biofilm proteins recognized by salivary immunoglobulin A.  Oral Microbiol. Immunol. 24:361-368.

Fontana M., D. Catt, G.J. Eckert, S. Ofner, M. Toro, R.L. Gregory, A. Ferreira Zandona, H. Eggertsson, R. Jackson, J. Chin, D. Zero and C. Sissons. 2009. Xylitol: effects on the acquisition of cariogenic species in infants.  J. Ped. Dent. 31:257-266.

Koh, I-W., Y. Oshida, C.J. Andres and R.L. Gregory.  2008. Effect of surface area ratios and bacteria on electrochemical behavior of galvanically coupled titanium.  Int. J. Prosthodont. 21:433-436.

Catt, D.M. and R.L. Gregory. 2005. Streptococcus mutans murein hydrolase.  J. Bact. 187:7863-7865.

Fugaro, O.J., J.O. Fugaro, B. Matis, R.L. Gregory, M.A. Cochran and I.A. Mjor. 2005. The dental pulp: inflammatory markers and vital bleaching. Am. J. Dent. 18:229-232.

Olson, B.L., N.A. Warner, R.L. Gregory, J.L. McDonald and C.A. Zapata. 2005. Periodontal ligament fibroblast interleukin-6 release after exposure to nicotine. Oral Biosciences Med. 2:15-19.

Ge, J., D.M. Catt and R.L. Gregory. 2004. Streptococcus mutans surface a-enolase binds salivary MG2 and human plasminogen. Infect. Immun. 72:6748-6752.

Chang, J-C., Y. Oshida, R.L. Gregory, C.J. Andres, T.M. Barco and D. Brown. 2003. Electrochemical study on microbiology-related corrosion of metallic materials.  Bio-Med. Mater. Eng. 13:281-295.

 Dowling, J.S., M. Hockenberry and R.L. Gregory. 2003. Sense of humor, childhood cancer stressors, and outcomes of psychosocial adjustment, immune function, and infection. J. Ped. Oncol. Nursing 20:271-292.

Wood, K.L., P. Chaiyarit, R.B. Day, Y. Wang, C.T. Schnizlein-Bick, R.L. Gregory and H.L. Twigg III. 2003. Measurements of HIV viral loads from different levels of the respiratory tract. Chest 124:536-542.

Gonzalez-Cabezas, C., Y. Li, R.L. Gregory and G.K. Stookey.  2002  Distribution of cariogenic bacteria in carious lesions around resin restorations.  Amer. J. Dent. 15:248-251.

Sanders, B.J., R.L. Gregory, K. Moore and D.R. Avery. 2002. Antibacterial and physical properties of resin modified glass-ionomers combined with chlorhexidine. J. Oral Rehabilitation 29:553-558.

Gregory, R.L. 2001. Modified immunogenicity of a mucosally administered antigen. Clin. Diagn. Lab. Immunol. 8:540-544.

Gregory, R.L. 1994. Dental caries vaccines: science and status. Compendium: The Compendium of Continuing Education in Dentistry 15:1282-1294.

Rose, P.T., R.L. Gregory, L.E. Gfell and C.V. Hughes. 1994. IgA antibodies to Streptococcus mutans in caries-resistant and -susceptible children. Pediatric Dent. 16:272-275.

Gregory, R.L., N.A. Warner, L.E. Gfell and M. Fontana. 1994. Visualization and characterization ofStreptococcus mutans fimbriae by negative and immunogold bead staining in the TEM. Microscopy Research and Technique 27:352.

Gregory, R.L. 1994. The biological role and clinical implications of IgA. Laboratory Medicine25:724-728.

Wilson, T.G. and R.L. Gregory. 1995. Clinical effectiveness of fluoride-releasing elastomers. I: SalivaryStreptococcus mutans numbers. American Journal of Orthodontics and Dentofacial Orthopedics 107:293-297.

Fontana, M., L.E. Gfell and R.L. Gregory. 1995. Characterization of preparations enriched forStreptococcus mutans fimbriae: salivary IgA antibodies in caries-free and caries-active subjects. Clin. Diagn. Lab. Immunol. 2:719-725.

Dung, S-Z., R.L. Gregory, Y. Li and G.K. Stookey. 1995. Effect of lactic acid and proteolytic enzymes on the release of organic matrix components from human root dentin. Caries Res. 29:483-489.

Gonzalez-Cabezas, C., Y. Li, T.W. Noblitt, R.L. Gregory, A.H. Kafrawy and G.K. Stookey. 1995. Detection of mutans streptococci in secondary caries lesions using immunofluorescent techniques and confocal laser scanning microscopy. Caries Res. 29:198-203.

Gregory, R.L. 1995. Diagnosis and role of Helicobacter pylori in gastrointestinal diseases. Clin. Lab. Sci.8:209-211.

Fontana, M., A.J. Dunipace, R.L. Gregory, T.W. Noblitt, Y. Li, K.K. Park and G.K. Stookey. 1996. An in vitro microbial model for studying secondary caries formation. Caries Res. 30:112-118.

Gregory, R.L. and L.E. Gfell. 1996. Effect of nicotine on secretory component synthesis by secretory epithelial cells. Clin. Diagn. Lab. Immunol. 3:578-583.

Perrone, M., L.E. Gfell, M. Fontana and R.L. Gregory. 1997. Antigenic characterization of fimbriae preparations from Streptococcus mutans isolates from caries-free and caries susceptible subjects. Clin. Diagn. Lab. Immunol. 4:291-296.

Pascale, J.M., M.D. Isaacs, P. Contreras, B. Gomez, L. Lozano, E. Austin, M.C. de Martin, R.L. Gregory, G.L. McLaughlin and A. Amador. 1997. Immunological markers of disease progression in patients infected with the human immunodeficiency virus. Clin. Diagn. Lab. Immunol. 4:474-477.

Gregory, R.L., J.P. Wallace, L.E. Gfell, J. Marks and B.A. King. 1997. Effect of exercise on milk immunoglobulin A levels. Med. Sci. Sports Exerc. 29:1596-1601.

Moore, M.A., R.L. Gregory, L. Switalski, Z.W. Hakki, L.E. Gfell and M.J. Kowolik. 1998. Differential activation of human neutrophils by Streptococcus mutans isolates from root surface lesions and caries free and caries active subjects. Oral Microbiol. Immunol. 13:41-46.

Gregory, R.L., A.M.A. Rahman and D.R. Avery. 1998. Effect of restorative treatment on mutans streptococci and IgA antibodies. Ped. Dent. 20:273-277.

Pascale, J.M., M.M. Shaw, P.J. Durant, A.A. Amador, M.S. Bartlett, J.W. Smith, R.L. Gregory and G.L. McLaughlin. 1999. Intranasal immunization confers protection against murine Pneumocystis carnii lung infection. Infect. Immun. 67:805-809.

Ray, C.A., L.E. Gfell, T.L. Buller and R.L. Gregory. 1999. Interactions of Streptococcus mutansfimbria-associated surface proteins with salivary components. Clin. Diagn. Lab. Immunol.6:400-404.

Fontana, M., A.J. Dunipace, G.K. Stookey and R.L. Gregory. 1999. Intranasal immunization against dental caries with Streptococcus mutans enriched fimbrial preparation. Clin. Diagn. Lab. Immunol. 6:405-409.

Chaiyarit, P., A.H. Kafrawy, D.A. Miles, S.L. Zunt, M.L. Van Dis and R.L. Gregory. 1999. Oral lichen planus: an immunohistochemical study of heat shock proteins (HSPs) and cytokeratins (CKs) and a unifying hypothesis of pathogenesis. J. Oral Pathol. Med. 28:210-215.

Gonzalez-Cabezas, C., Y. Li, R.L. Gregory and G.K. Stookey. 1999. Distribution of three cariogenic bacteria in secondary carious lesions around amalgam restorations. Caries Res. 33:357-365.

Fontana, M., T.L. Buller, A.J. Dunipace, G.K. Stookey and R.L. Gregory. 2000. An in vitro microbial-caries model used to study the efficacy of antibodies to Streptococcus mutanssurface proteins in preventing dental caries. Clin. Diagn. Lab. Immunol. 7:49-54.

Toro, M.J., L. Escalona, P. Chaiyarit, N.A. Warner and R.L. Gregory. 2001. Salivary immunoglobulin A antibodies to gp41 in human immunodeficiency virus-seropositive patients: lack of correlation with disease progression.  Oral Microbiol. Immunol. 16:188-192.

Gregory, R.L. 2001. Modified immunogenicity of a mucosally administered antigen. Clin. Diagn. Lab. Immunol. 8:540-544.

Sanders, B.J., R.L. Gregory, K. Moore and D.R. Avery. 2002. Antibacterial and physical properties of resin modified glass-ionomers combined with chlorhexidine. J. Oral Rehabilitation 29:553-558.

Gonzalez-Cabezas, C., Y. Li, R.L. Gregory and G.K. Stookey.  2002  Distribution of cariogenic bacteria in carious lesions around resin restorations.  Amer. J. Dent. 15:248-251.

Wood, K.L., P. Chaiyarit, R.B. Day, Y. Wang, C.T. Schnizlein-Bick, R.L. Gregory and H.L. Twigg III. 2003. Measurements of HIV viral loads from different levels of the respiratory tract. Chest 124:536-542.

Chang, J-C., Y. Oshida, R.L. Gregory, C.J. Andres, T.M. Barco and D. Brown. 2003. Electrochemical study on microbiology-related corrosion of metallic materials.  Bio-Med. Mater. Eng. 13:281-295. Dowling, J.S., M. Hockenberry and R.L. Gregory. 2003. Sense of humor, childhood cancer stressors, and outcomes of psychosocial adjustment, immune function, and infection. J. Ped. Oncol. Nursing 20:271-292.

Ge, J., D.M. Catt and R.L. Gregory. 2004. Streptococcus mutans surface a-enolase binds salivary MG2 and human plasminogen. Infect. Immun. 72:6748-6752.

 

*select recent publications out of 278 total refereed publications and abstracts